Development of a piezoelectric immunosensor for the measurement of paclitaxel.
Identifieur interne : 001813 ( Main/Exploration ); précédent : 001812; suivant : 001814Development of a piezoelectric immunosensor for the measurement of paclitaxel.
Auteurs : L. Pastorino [Italie] ; F. Caneva Soumetz ; M. Giacomini ; C. RuggieroSource :
- Journal of immunological methods [ 0022-1759 ] ; 2006.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (chemistry), Antibodies, Monoclonal (immunology), Biosensing Techniques (methods), Calibration, Cattle, Microscopy, Atomic Force, Paclitaxel (analysis), Paclitaxel (immunology), Polyethylenes (chemistry), Polystyrenes (chemistry), Quartz (chemistry), Quaternary Ammonium Compounds (chemistry), Serum Albumin, Bovine (chemistry), Surface Properties, Taxoids (immunology).
- MESH :
- chemical , analysis : Paclitaxel.
- chemical , chemistry : Antibodies, Monoclonal, Polyethylenes, Polystyrenes, Quartz, Quaternary Ammonium Compounds, Serum Albumin, Bovine.
- chemical , immunology : Antibodies, Monoclonal, Paclitaxel, Taxoids.
- methods : Biosensing Techniques.
- Animals, Calibration, Cattle, Microscopy, Atomic Force, Surface Properties.
Abstract
This paper describes the development of a piezoelectric immunosensor for the measurement of paclitaxel (taxol), a natural anti-cancer agent. An antibody specific for taxanes was immobilized onto the surface of quartz crystals by means of the layer-by-layer self-assembly technique. The immobilization was achieved using electrostatic interactions between a precursor layer and the antibody molecules. The assembly process was monitored by a quartz crystal microbalance (QCM) and the topography of the modified quartz crystals was investigated by means of atomic force microscopy. The specific interaction of the immobilized antibody with paclitaxel in solution at different concentrations was monitored as a change in resonant frequency of the modified crystal. Moreover, the influence of non-specific adsorption was also characterized. The results show that the proposed immunosensor offers a promising alternative to classical analytical methods for a fast and easy determination of paclitaxel.
DOI: 10.1016/j.jim.2006.05.003
PubMed: 16774765
Affiliations:
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Le document en format XML
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Giacomini</name></author><author><name sortKey="Ruggiero, C" sort="Ruggiero, C" uniqKey="Ruggiero C" first="C" last="Ruggiero">C. Ruggiero</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="doi">10.1016/j.jim.2006.05.003</idno><idno type="RBID">pubmed:16774765</idno><idno type="pmid">16774765</idno><idno type="wicri:Area/PubMed/Corpus">000487</idno><idno type="wicri:Area/PubMed/Curation">000487</idno><idno type="wicri:Area/PubMed/Checkpoint">000472</idno><idno type="wicri:Area/Ncbi/Merge">000397</idno><idno type="wicri:Area/Ncbi/Curation">000397</idno><idno type="wicri:Area/Ncbi/Checkpoint">000397</idno><idno type="wicri:doubleKey">0022-1759:2006:Pastorino L:development:of:a</idno><idno type="wicri:Area/Main/Merge">001846</idno><idno type="wicri:Area/Main/Curation">001813</idno><idno type="wicri:Area/Main/Exploration">001813</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development of a piezoelectric immunosensor for the measurement of paclitaxel.</title><author><name sortKey="Pastorino, L" sort="Pastorino, L" uniqKey="Pastorino L" first="L" last="Pastorino">L. Pastorino</name><affiliation wicri:level="1"><nlm:affiliation>Department of Communication, Computer and System Sciences, University of Genoa, Via Opera Pia, 13-16145 Genova, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Communication, Computer and System Sciences, University of Genoa, Via Opera Pia, 13-16145 Genova</wicri:regionArea><wicri:noRegion>13-16145 Genova</wicri:noRegion></affiliation></author><author><name sortKey="Caneva Soumetz, F" sort="Caneva Soumetz, F" uniqKey="Caneva Soumetz F" first="F" last="Caneva Soumetz">F. Caneva Soumetz</name></author><author><name sortKey="Giacomini, M" sort="Giacomini, M" uniqKey="Giacomini M" first="M" last="Giacomini">M. Giacomini</name></author><author><name sortKey="Ruggiero, C" sort="Ruggiero, C" uniqKey="Ruggiero C" first="C" last="Ruggiero">C. Ruggiero</name></author></analytic><series><title level="j">Journal of immunological methods</title><idno type="ISSN">0022-1759</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (chemistry)</term><term>Antibodies, Monoclonal (immunology)</term><term>Biosensing Techniques (methods)</term><term>Calibration</term><term>Cattle</term><term>Microscopy, Atomic Force</term><term>Paclitaxel (analysis)</term><term>Paclitaxel (immunology)</term><term>Polyethylenes (chemistry)</term><term>Polystyrenes (chemistry)</term><term>Quartz (chemistry)</term><term>Quaternary Ammonium Compounds (chemistry)</term><term>Serum Albumin, Bovine (chemistry)</term><term>Surface Properties</term><term>Taxoids (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Paclitaxel</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Polyethylenes</term><term>Polystyrenes</term><term>Quartz</term><term>Quaternary Ammonium Compounds</term><term>Serum Albumin, Bovine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Paclitaxel</term><term>Taxoids</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Calibration</term><term>Cattle</term><term>Microscopy, Atomic Force</term><term>Surface Properties</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This paper describes the development of a piezoelectric immunosensor for the measurement of paclitaxel (taxol), a natural anti-cancer agent. An antibody specific for taxanes was immobilized onto the surface of quartz crystals by means of the layer-by-layer self-assembly technique. The immobilization was achieved using electrostatic interactions between a precursor layer and the antibody molecules. The assembly process was monitored by a quartz crystal microbalance (QCM) and the topography of the modified quartz crystals was investigated by means of atomic force microscopy. The specific interaction of the immobilized antibody with paclitaxel in solution at different concentrations was monitored as a change in resonant frequency of the modified crystal. Moreover, the influence of non-specific adsorption was also characterized. The results show that the proposed immunosensor offers a promising alternative to classical analytical methods for a fast and easy determination of paclitaxel.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Caneva Soumetz, F" sort="Caneva Soumetz, F" uniqKey="Caneva Soumetz F" first="F" last="Caneva Soumetz">F. Caneva Soumetz</name><name sortKey="Giacomini, M" sort="Giacomini, M" uniqKey="Giacomini M" first="M" last="Giacomini">M. Giacomini</name><name sortKey="Ruggiero, C" sort="Ruggiero, C" uniqKey="Ruggiero C" first="C" last="Ruggiero">C. Ruggiero</name></noCountry><country name="Italie"><noRegion><name sortKey="Pastorino, L" sort="Pastorino, L" uniqKey="Pastorino L" first="L" last="Pastorino">L. Pastorino</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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